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Roland Zengerle is full professor at the Department of Microsystems Engineering at the University of Freiburg, director at the “Hahn-Schickard-Institut für Mikroanalysesysteme”.
DARQ CHAPTER 4 REGISTRATION
He leads the Regulatory Affairs Team at Mast and is responsible for product registration worldwide.
DARQ CHAPTER 4 ISO
He works as a Medical Product Safety Manager in the terms of the EN ISO 13485 standard and the German Medical Product Law. He is leading a team for the development of molecular diagnostic (based on the LAMP) and protein technologies. Sieghard Frischmann joined Mast in 1992 and now he is head of product development and production at Mast. Since 2014, he has been working for the product development team of Mast Diagnostica GmbH using LAMP.ĭr Sieghard Frischmann studied biology at the Friedrich-Alexander-Universität Erlangen and completed his PhD at the University of Hamburg, Universitätsklinik Eppendorf. In his second postdoc term at Research Center Borstel he participated further in development of isothermal diagnostics for tick-borne diseases.
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As a postdoc, he worked in diagnostics (both serological and molecular), first in the National Reference Center for Protozoan Diseases (NRCPD), Obihiro University focusing on methods for the diagnosis of Cryptosporidium species infection using LAMP.
DARQ CHAPTER 4 FREE
Her current research is focused on real-time and digital assays, isothermal amplification and single cell analysis on lab-on-a-chip platforms.ĭr Mohammed Bakheit obtained his Bachelor and Master's degrees in the Faculty of Veterinary Medicine, University of Khartoum and doctoral degree in the Free University of Berlin. In 2016, Nadine Borst joined Hahn-Schickard and since February 2017 she is heading the Nucleic Acid Analysis group. There, she worked in the field of enzyme engineering and assay development. Her research involves nucleic acid analysis with focus on isothermal amplification and digital amplification on centrifugal microfluidic cartridges.ĭr Nadine Borst studied biochemistry at the University of Regensburg and completed her PhD at the Technical University of Munich in cooperation with the Fraunhofer Institute for Interfacial Engineering and Biotechnology. She currently works as a PhD candidate at Hahn-Schickard and at the Laboratory for MEMS Applications at IMTEK, University of Freiburg. In 2015 she graduated as a Master of Science with a master's thesis on spin–spin interactions on DNA aptamer complexes. Lisa Becherer studied chemistry at the University of Freiburg. In summary, recent developments in methods for the sequence-specific detection of LAMP demonstrate high potential for many future applications. We also observe an increase in the use of smartphone-integrated sensors to improve LAMP-based point-of-need testing. In addition, the technological readiness level and the kind of platform (open versus method-tailored) are evaluated, the latter playing an important role in the miniaturization and automation of operational process steps. Instrumental requirements are discussed in terms of complexity, portability and fluidic cartridges. Fluorescence-based detection, making up half of the methods, can be processed on open platforms and satisfies all the criteria listed before.
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To give structure to the diverse multitude of sequence-specific methods, we created a systematic classification and provide a critical comparative evaluation according to a catalogue of criteria (analytical performance, multiplexing, quantification and instrumental requirements). In the last 13 years, a variety of sequence-specific methods have emerged, based on a very diverse range of sensing techniques, including optical, magnetic, piezoelectric, electrochemical and magnetoresistive sensing. Recently, methods for sequence-specific detection have gained more importance because, unlike sequence-independent detection methods, they are highly specific towards the target DNA. Moreover, LAMP excels through its isothermal and energy efficient amplification requirements, rendering it a prime candidate for low-cost diagnostics and analysis at the point of need. Loop-mediated isothermal amplification (LAMP) is well known for its robust and highly sensitive and specific amplification of target DNA, which is achieved by utilizing up to six primers. In the course of the last 20 years, isothermal nucleic acid amplification tests have emerged as an important diagnostic tool, not only for clinical applications, but also for food quality control and environmental monitoring.